bio safe cbb g 250 stain  (Bio-Rad)

Journal: Journal of Clinical Microbiology

Article Title: Development and validation of enzyme-linked immunosorbent assays for the serodiagnosis of canine bartonelloses

doi: 10.1128/jcm.00267-25

Figure Lengend Snippet: Coomassie-stained SDS-PAGE and WB analysis of purified recombinant proteins (rATP-β, rGroEL, rLemA, rSucB, and rVirB5). WB analysis of purified proteins was performed using mouse anti-His antibody (Thermo Scientific, Rockford, IL) and alkaline-phosphatase-conjugated Goat anti-mouse IgG (Thermo Scientific, Rockford, IL). Predicted molecular mass of recombinant proteins was determined by the coding sequence of the specified gene insert plus fused in-frame with pET200D/TOPO expression system fusion tag (~3 kDa) as indicated by red arrows. Green arrows indicate recombinant proteins in multimeric forms. Coomassie, Coomassie-stained SDS-PAGE; Anti-His, WB analysis; and L, Bio Rad Precision Plus Protein Kaleidoscope Prestained Protein Standard.

Article Snippet: Fractionated proteins were visualized by staining the gel overnight with Bio-safe Coomassie brilliant blue (Bio-Rad, Hercules, CA).

Techniques: Staining, SDS Page, Purification, Recombinant, Sequencing, Expressing

Journal: bioRxiv

Article Title: Endocytosis of the damage-associated molecular pattern receptor PEPR1 is BAK1-dependent

doi: 10.1101/2025.03.15.643409

Figure Lengend Snippet: The cytoplasmic domains of BAK1 S612A , BAK1 AAA , BAK1-5, BAK1, and PEPR1, and their respective kinase-dead mutant (Km) versions of PEPR1-Km (K855E) and BAK1-Km (D416N) tagged with either glutathione S-transferase (GST) or His6-maltose-binding protein (MBP) were produced in bacteria. In vitro kinase assay performed with a single or combined recombinant proteins as indicated. CBB, Coomassie Brilliant Blue.

Article Snippet: The gels were stained with Bio-Safe Coomassie Brilliant Blue (CBB) (Bio-Rad), dried between cellophane plastic sheets, and [γ- 32 P] was detected by autoradiography.

Techniques: Mutagenesis, Binding Assay, Produced, Bacteria, In Vitro, Kinase Assay, Recombinant

Journal: bioRxiv

Article Title: Endocytosis of the damage-associated molecular pattern receptor PEPR1 is BAK1-dependent

doi: 10.1101/2025.03.15.643409

Figure Lengend Snippet: For the in vitro kinase assay a single or recombinant proteins together were used, as indicated. Protein loading was controlled by Coomassie Brilliant Blue (CBB).

Article Snippet: The gels were stained with Bio-Safe Coomassie Brilliant Blue (CBB) (Bio-Rad), dried between cellophane plastic sheets, and [γ- 32 P] was detected by autoradiography.

Techniques: In Vitro, Kinase Assay, Recombinant